酚磺乙胺对酶法检测血清肌酐的影响

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Abstract Objective To investigate the effect of Ethamsylate on the enzymatic detection of serum creatinine(CREA).Methods Fresh serum was collected from patients who came to Guizhou Provincial People′s Hospital in October 2019 and prepared the collected serum to be high and low concentrations of CREA mixed serum.The interference screening experiments and dose-effect experiments were conducted on enzymatic detection of serum CREA according to the EP7-A2 document developed by the Clinical and Laboratory Standards Institute(CLSI).Then the interfering results at different concentrations were used to determine the effect of Ethamsylate on detection of CREA.Results Interference screening experiment：the average value of the experimental group sample was 166.28μmol/L,and that of the control group was 403.44μmol/L.The interference value (dabs)95%CI of the experimental group was(-237.16±1.82)μmol/L,indicated the lowest offline value of the dabs 95%CI was greater than the acceptable standard(dmax)of the preset interference experiment.Dose-effect experiment：the regression curve equation of interference effect of enzymatic on low value CREA samples was Y=-1.727e-6X3+0.0016X2-0.474X-5.285(F=406.080,R2=0.981,P=0.000),when the concentration of ethamsylate was below 1.174 mg/L,the dabs was lower than the dmax for interference experiment.Meanwhile,the regression curve equation of Ethamsylate for high value CREA samples was Y=-5.105e-6X3+0.00499X2-1.632X-11.252(F=1483.642,R2=0.995，P=0.000),and the dabs was lower than the dmax when the concentration of Ethamsylate was below 9.727 mg/L.Conclusion Ethamsylate causes the negative interference on the serum CREA detection by enzymatic method which may be attribute to the factors of detection principle.It is suggest to consult the patients whether to take Ethamsylate drug,redraw blood after discontinuation of the drug,or use other principles of CREA assay to check if necessary.Meanwhile,this study also provides some references for the accurate analysis of CREA.

Key words： Ethamsylate Enzymatic method Serum creatinine Drug interference

Received: 06 February 2020

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	JING Hang1 NIU Zhi-jie1 LIU Zi-yan1 LIU Lin2 CHEN Sheng-chao3 WU Hong-quan1▲

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JING Hang1 NIU Zhi-jie1 LIU Zi-yan1 LIU Lin2 CHEN Sheng-chao3 WU Hong-quan1▲. Effect of Ethamsylate on the enzymatic detection of serum creatinine[J]. 中国当代医药, 2020, 27(24): 9-12.

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https://www.dangdaiyiyao.com/EN/ OR https://www.dangdaiyiyao.com/EN/Y2020/V27/I24/9

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