温阳散纤汤对博来霉素诱导的肺间质纤维化小鼠肺组织中羟脯氨酸及转化生长因子-β1含量的影响

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Abstract Objective To explore effect of Wenyang Sanxian Decoction on the contents of hydroxyproline (Hyp) and transforming growth factor-β1(TGF-β1)in lung tissue of mice with pulmonary interstitial fibrosis induced by Bleomycin.Methods Bleomycin (0.2-0.3 ml, 5 mg/kg) was injected into C57BL/6 mice through the trachea to establish a pulmonary interstitial fibrosis model. The successful 18 pulmonary fibrosis mice were randomly divided into model group,Wenyang Sanxian Decoction low, medium and high dose groups [5.6625, 11.3250, 22.6500 mg/(kg·d)], positive group[15 mg/(kg·d) Methylprednisolone], with 3 mice in each group. In addition, sham operation and normal group were established, with 3 mice in each group. The contents of Hyp and TGF-β1 in lung tissue were detected by enzyme linked immunosorbent assay (ELISA). Results The contents of Hyp and TGF-β1 in the model group were higher than those in the normal group and sham group, the differences were statistically significant (P＜0.05). The contents of Hyp and TGFβ1 in the Wenyang Sanxian Decoction low, medium and high dose groups and positive group were lower than those in the model group, the differences were statistically significant (P ＜0.05). The contents of Hyp and TGF-β1 in the Wenyang Sanxian Decoction middle, high dose groups and positive group were higher than those in the Wenyang Sanxian Decoction low dose group, the differences were statistically significant (P＜0.05). The contents of Hyp and TGF-β1 in the Wenyang Sanxian Decoction high dose group and positive group were lower than those in the Wenyang Sanxian Decoction middle dose group, the differences were statistically significant (P＜0.05). Conclusion A certain dose of Wenyang Sanxian Decoction could improve the pulmonary interstitial fibrosis of C57BL/6 mice by reducing the contents of Hyp and TGF-β1.

Key words： Wenyang Sanxian Decoction Bleomycin Mice with pulmonary interstitial fibrosis Hydroxyproline Transforming growth factor-β1

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	ZHU Xiao-long
	QIU Nian-nian
	HU Qi-gui

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ZHU Xiao-long,QIU Nian-nian,HU Qi-gui. Effect of Wenyang Sanxian Decoction on the contents of hydroxyproline and transforming growth factor-β1 in lung tissue of mice with pulmonary interstitial fibrosis induced by Bleomycin[J]. 中国当代医药, 2020, 27(28): 4-7.

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https://www.dangdaiyiyao.com/EN/ OR https://www.dangdaiyiyao.com/EN/Y2020/V27/I28/4

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