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| Effect of lipoic acid on the expression of NLRP3 inflammasome in glomerular mesangial cells induced by high glucose |
| FENG Ying1 ZHAO Shumin1 WANG Zhongying1 LIU Yan2 |
1. Department of Internal Medicine Teaching and Research Office, Heze Medical College, Shandong Province, Heze 274000, China;
2. Department of Endocrinology, Affiliated Hospital of Heze Medical College, Shandong Province, Heze 274000, China |
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Abstract Objective To observe the effect of hyperglycemia on the expression of NLRP3 inflammasome in cultured human glomerular mesangial cells (HMC), and to explore the intervention mechanism of lipoic acid (LA) on the above process, so as to explore the new pathogenesis and prevention strategies of diabetic nephropathy (DN). Methods Human HMC was subcultured and divided into normal control group (NG), high glucose group (HG), LA group and HG+LA group according to different intervention factors. CCK-8 detects the effects of different concentrations of LA on HMC activity. Flow cytometry detects the level of reactive oxygen species (ROS), RT-PCR detects the expression of NLRP3 inflammasomes, and Western-blot detects the effect of LA on the protein expression of NLRP3 inflammasomes in HMC.Results CCK-8 results showed that the optimal protective concentration of LA was 200 μmol/L. The expression level of ROS in the HG group was higher than that in the NG group as detected by flow cytometry, and the difference was statistically significant (P＜0.05). RT-PCR and Western-blot test results showed that the expression of NLRP3 inflammasome in the HG group was higher than that in the NG group, and the difference was statistically significant (P＜0.05).After adding LA, the expression of NLRP3 inflammasome decreased, and the difference was statistically significant (P＜0.05). Conclusion A high glucose environment can promote the proliferation of HMC. Within a certain concentration range, lipoic acid at an appropriate concentration can inhibit the proliferation of HMC; hyperglycemia can increase the expression of NLRP3 inflammasomes through oxidative stress and affect the occurrence and development of diabetic nephropathy. The lipoic acid has an inhibitory effect on the above process.
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