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| Optimization of extraction technology of 1-deoxynojirimycin from mulberry seeds by orthogonal test |
| LYU Zhiqiang1 LI Qiao2 YAN Hailong3 XU Wen1 ZHAO Zhenhuan1 GONG Lili4 TIAN Jingzhen4▲ |
1. Pharmaceutical Department, the Affiliated Hospital of Qingdao University, Shangdong Province, Qingdao 266000,China;
2. Outpatient Department of General Affairs Department, Qingdao Vocational and Technical College of Hotel Management, Shangdong Province, Qingdao 266000, China;
3. Rehabilitation Department, Qingdao Women and Children's Hospital, Shangdong Province, Qingdao 266000, China;
4. College of Pharmacy, Shandong University of TCM,Shangdong Province, Jin'an 250355, China |
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Abstract Objective To optimize the extraction process of 1-deoxynojirimycin (DNJ) from mulberry seeds by orthogonal test. Methods The extraction rate of DNJ from mulberry seeds was determined by precolumn derivatization high performance liquid chromatography (HPLC). Based on single factor experiment, the effects of extraction temperature,ethanol concentration and hydrochloric acid concentration on the extraction rate of DNJ from mulberry seeds were investigated. The optimum extraction process of DNJ from mulberry seed was determined by orthogonal test. Results Single factor experiment was conducted to investigate and analyze the extraction time, solvent amount, extraction times,etc., the differences were not statistically significant (P＞0.05). The orthogonal experimental variance analysis of hydrochloric acid concentration, ethanol concentration and extraction temperature showed that the hydrochloric acid concentration had significant effect on the extraction effect of DNJ, the difference was statistically significant (P＜0.05).Considering all factors, the best extraction scheme is determined as follows: hydrochloric acid concentration 0.05 mol/L,ethanol concentration 50%, extraction temperature 70℃. Under these conditions, the extraction rate of DNJ was 0.59%.Conclusion Under the conditions of hydrochloric acid concentration of 0.05 mol/L, ethanol concentration of 50% and extraction temperature of 70℃, the extraction rate of DNJ from mulberry seeds is the highest. This method is simple and can be used as the extraction process of DNJ from mulberry seeds.
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