抗风湿镇痛类健康产品中吲哚美辛的快速筛查方法

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Abstract Objective To establish a rapid screening method for the detection of indometacin in antirheumatic pain relieving healthy products. Methods The samples of different dosage forms were extracted with 4 ml of 0.4% sodium hydroxide solution, acidified and precipitated impurities with 2 ml of 3 mol/L sulfuric acid solution. The filtrate was added with 1 drop of 5% ferric chloride solution and 3% hydrogen peroxide solution for color reaction. The confirmation of samples by high performance liquid chromatography-tandem mass spectrometry (LC-MS) was performed on a CAPCELL PAK C18 MG Ⅲ(2.0 mm×150 mm, 5 μm) column, with gradient elution of 0.02 mol/L ammonium acetateacetonitrile, the flow rate was 0.2 ml/min, the column temperature was 35°C, the injection volume was 10 μl, and the detection was multiple reaction monitoring (MRM) in negative ion mode. Results A total of 112 batches of samples of different dosage forms (27 batches of tablets, 5 batches of granules, 61 batches of capsules and 19 batches of pills)were collected by chromogenic reaction method for rapid screening. Sixteen batches of positive samples were detected(capsule 15 batches, pill 1 batch), the detection limit of method was 0.25 mg/times, the rate of missed detection and error detection was 0%, and the correct rate was 100%. Conclusion Color reaction has strong specificity, low missed detection rate and low false detection rate, and high accuracy. It can be employed in the rapid screening detection of Indometacin in antirheumatic pain relieving health products.

Key words： Antirheumatic pain relieving healthy products Indomethacin Color reaction method Rapid screening;High performance liquid chromatography tandem mass spectrometry

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	Articles by authors
	WEI Zhixiong
	LIU Dandan
	LAN Mingxiong
	WANG Kunpeng

Cite this article:

WEI Zhixiong,LIU Dandan,LAN Mingxiong, et al. Detection of Indometacin in antirheumatic pain relieving health products by rapid screening method[J]. 中国当代医药, 2022, 29(4): 41-44.

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https://www.dangdaiyiyao.com/EN/ OR https://www.dangdaiyiyao.com/EN/Y2022/V29/I4/41

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