早期母爱剥夺影响边缘系统-丘脑-皮质环路致大鼠成年后抑郁的机制研究

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Abstract Objective To investigate the effects of maternal deprivationon neuron and expression of GFAP in prefrontal,hippocampus and thalamus, which are important components of limbic-thalamic cortical circuitry, and to explore the pathological mechanisms of maternal deprivationcausing adult depression-like manifestations. Methods Newborn rats were divided into two groups: maternal deprivation group (n=20) and control group (n=20) by random number table method. Rats in maternal deprivationgroup were exposed to maternal deprivation (3 hours per day) from postnatal day 1 to 14 while rats in control group were not. The sucrose preference and sociability tests were conducted when the rats were two months old. Then all the rats were sacrificed and their brains were collected, and the immunofluorescence staining were applied on Neun and GFAP. The confocal microscope was used for observation and photography, and the ImageJ was used for the number of positive cells and statistical analysis. Results Compared with the control group, the maternal deprivation group showed depression-like behaviors such as anhedonia and reduced social initiative, and the number of Neun positive cells in the prefrontal lobe,hippocampus and thalamus in the maternal deprivation group was less than that in the control group, with statistical significance (P<0.05). The results of GFAP immunofluorescence staining showed that the number of GFAP positive cells in the prefrontal lobe, hippocampus and thalamus in the maternal deprivation group was lower than that in the control group, and the difference was statistically significant (P<0.05). Conclusion Maternal deprivation can cause to depressed behaviors in adult rats and downregulates the numbers of neuron and astrocyte in prefrontal, hippocampus and thalamus-important parts of limbic-thalamic-cortical circuitry that might be involved in the mechanisms of maternal deprivationcaused adult depression.

Key words： Maternal deprivation Limbic-thalamic-cortical circuitry Depression Astrocyte

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	PAN Nannan
	LU Haoyang
	ZHOU Liemin
	NING Yuping
	FANG Ziyan

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PAN Nannan,LU Haoyang,ZHOU Liemin, et al. Study on influences of early life maternal deprivation on limbic-thalamic-cortical circuitry which are associated with depression in adult rats[J]. 中国当代医药, 2022, 29(6): 7-10转19.

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https://www.dangdaiyiyao.com/EN/ OR https://www.dangdaiyiyao.com/EN/Y2022/V29/I6/7

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