肿瘤抑素19肽对SK-Hep-1细胞增殖和凋亡作用的影响

Abstract
Figure/Table
References (16)
Related Citation (15)

Download: PDF (1037 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To study the effect of tumstatin 19 peptide proliferation and apoptosis of hepatoma SK-Hep-1 cells. Methods CCK-8 was used to detect the effect of 19 peptide （0, 50, 100, 150, 200, 250 μg/ml） on the proliferation of SK-Hep-1 cells. DAPI staining was used to detect the morphological changes of SK-Hep-1 cell apoptosis,and flow cytometry was used to detect the effect of 19 peptide on SK-Hep-1 cell apoptosis. Results Sk-hep-1 cells were treated with 0, 50, 100, 150, 200, 250 μg/ml 19 peptide, after 24 h, the survival rates were (100.00±1.80)%, (95.64±1.92)%, (82.18±1.67)%, (68.43±1.39)%, (46.74±1.35)%, (42.49±1.85)%, respectively. After 48 h treatment, the cell survival rate was (100.00±1.80)%, (87.27±1.96)%, (66.32±1.74)%, (58.78±1.94)%, (40.40±1.44)%, (22.15±1.51)%, respectively. When 19 peptide was 0 μg/ml, most of SK-HEP-1 cells were stained uniformly and blue, and apoptosis did not occur. Compared with 0 μg/ml, some cells showed bright blue after treated with 50, 100, 150, 200 and 250 μg/ml 19 peptide for 24 h, and the nuclei showed concentration, rupture, fragmentary dense and dense staining or cap-like apoptotic morphology. The apoptosis rate increased with the increase of 19 peptide concentration. Conclusion 19 peptide can effectively inhibit the proliferation of SK-Hep-1 cells and induce the apoptosis, which provides theoretical guidance for the research and clinical treatment of new anti hepatoma drugs.

Key words： Tumstatin 19 peptide Proliferation Apoptosis Liver cancer

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Shun
	LIU Taoyuan
	LI Ang
	YI Tonghui
	PAN Hongming
	LI Shuyan▲

Cite this article:

WANG Shun,LIU Taoyuan,LI Ang, et al. Effect of tumstatin 19 peptide on proliferation and apoptosis of SK-Hep-1 cells[J]. 中国当代医药, 2022, 29(2): 42-44.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2022/V29/I2/42

[1]	Craig AJ，von Felden J，Garcia-Lezana T，et al.Tumour evolution in hepatocellular carcinoma[J].Nat Rev Gastroenterol Hepatol，2020，17（3）：139-152.
[2]	Llovet JM，Zucman-Rossi J，Pikarsky E，et al.Hepatocellular carcinoma[J].Nat Rev Dis Primers，2016，14（2）：16018-16023.
[3]	张浩，卢立杰，潘佳佳.TACE 联合塞来昔布和醋酸奥曲肽对中期及进展期肝癌疗效及预后的影响[J].重庆医学，2021，21（1）：1-8.
[4]	张溪，弓磊.抗菌肽抗菌机制及研究热点[J].中国组织工程研究，2020，24（10）：1634-1640.
[5]	Ucak I，Afreen M，Montesano D，et al.Functional and Bioactive Properties of Peptides Derived from Marine Side Streams[J].Mar Drugs，2021，19（2）：71-77.
[6]	罗月，薛宏伟，王培军.应重视肝纤维化的影像学研究进展及应用[J].中华医学杂志，2019，99（7）：481-483.
[7]	李瑭，熊雁，蔡磊，等.肿瘤抑素T7 肽溶液的稳定性研究[J].中国药剂学杂志，2018，16（6）：101-108.
[8]	Hamano Y，Zeisberg M，Sugimoto H，et al.Physiological levels of tumstatin，a fragment of collagen Ⅳalpha3 chain，are generated by MMP-9 proteolysis and suppress angiogenesis via alpha Ⅴbeta3 integrin[J].Cancer Cell，2003，3（6）：589-601.
[9]	Maeshima Y，Colorado PC，Torre A，et al.Distinct antitumor properties of a type Ⅳcollagen domain derived from basement membrane[J].J Biol Chem，2000，275（28）：21340-21348.
[10]	贺岩.肿瘤抑素19 肽对低分化胃癌细胞体内外抑瘤作用的研究[D].哈尔滨：哈尔滨医科大学，2007.
[11]	衣同辉，吴艳敏，刘睿，等.人参皂苷Rg3 联合肿瘤抑素19 肽对肝癌HepG2 细胞凋亡的影响及机制[J].北京中医药大学学报，2020，43（7）：575-582.
[12]	刘桂炎.LncRNA HOTAIR 调控通路遗传变异与肝癌易感性关联研究[D].广东药科大学，2020，21（1）：98-99.
[13]	吴坤，张迎春，邓思波，等.家蝇抗真菌肽-1A 对人肝癌细胞HepG2 的影响[J].贵州医科大学学报，2021，46（2）：125-130.
[14]	Rutherfurd-Markwick KJ.Food proteins as a source of bioactive peptides with diverse functions[J].Br J Nutr，2012，108（2）：149-157.
[15]	王淑静，张艺，刘佳，等.肿瘤抑素抗肿瘤活性肽的研究进展[J].中国肿瘤，2012，21（1）：56-59.
[16]	来庆娟，熊壮，徐晓浩，等.大黄素诱导肝癌细胞凋亡的研究进展[J].中华中医药学刊，2021，39（6）：129-132.