紫背天葵提取物对糖尿病小鼠血管炎症的影响

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Abstract Objective To observe the effect of extract of Begonia Fimbristipula on the vascular inflammation of diabetic mices. Methods A total of 60 healthy Kunming mice from June to August in 2019 were selected as the research objects. Six mice were randomly selected as normal control group and fed with normal diet. The other 54 mice were fed with high sugar and high fat diet for one month and induced by low-dose streptozotocin (STZ). Mice without successful modeling (blood glucose <11.0 mmol/L) were excluded, and the remaining successful modeling mice were divided into diabetic model group, different doses of Begonia Fimbristipula extract group and Metformin-positive control group, with 6 mice in each group. The extract group was given 0.75, 1.5 and 3 g/kg intragastric administration, the Metformin group was given 150 mg/kg intragastric administration, and the diabetic model group was given normal saline intragastric administration. After 4 weeks, fasting blood glucose, body weight and oral glucose tolerance were compared. The contents of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in serum of mice in each group were compared by enzyme-linked immunosorbent assay (ELISA). The expressions of IL-1β, TNF-α and IL-10 in thoracic aorta of mice were compared by real-time fluorescence quantitative PCR. Results At the end of the experiment, blood glucose, area under oral glucose tolerance curve, contents of IL-1β and TNF-α in serum and mRNA expression of IL-1β and TNF-α in vascular tissue in diabetic model group were higher than those in normal control group, and the differences were statistically significant (P<0.05). The body weight, serum IL-10 content and IL-10 mRNA expression in vascular tissues of dia-betic model group were lower than those of normal control group, and the differences were statistically significant (P<0.05). After the experiment, blood glucose, area under blood glucose curve, contents of IL-1β and TNF-α in serum and mRNA expression of IL-1β and TNF-α in vascular tissue in 1.5 and 3 g/kg Begonia Fimbristipula extract group and Metformin group were lower than those in diabetic model group, and the differences were statistically significant (P<0.05). The body weight, serum IL-10 content and IL-10 mRNA expression in blood vessels of mice in 1.5 and 3 g/kg Begonia Fimbristipula extract group and Metformin group were higher than those in diabetic model group, and the differences were statistically significant (P<0.05). Conclusion Extract of Begonia Fimbristipula can inhibit the production of vascular inflammation in diabetic mices.

Key words： Extract of Begonia Fimbristipula Diabetic mice Vascular inflammation Real-time fluorescence quantitative PCR

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	LIU Si-yu
	LI Yi
	TAN Hui
	HUANG Tian
	ZHANG Yu-qin
	GU Bin▲

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LIU Si-yu,LI Yi,TAN Hui, et al. Effect of extract of Begonia Fimbristipula on the vascular inflammation of diabetic mices[J]. 中国当代医药, 2021, 28(34): 4-8.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I34/4

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