天麻素对次黄嘌呤诱导小鼠急性高尿酸血症的影响和机制研究

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Abstract Objective To study the effects and possible mechanisms of gastrodin(GAS)on acute hyperuricemia(HUA)of mice induced by hypoxanthine.Methods A total of 50 male Kunming mice were randomly divided into five groups,which included the normal group,the model group,GAS 200 mg/kg group,GAS 400 mg/kg group,and allopurinol(ALLO)10 mg/kg group.There were 10 mice in each group,and the drugs were intragastricaly administered for 14 days.One hour after the last drug administration,mice were intraperitoneally injected with hypoxanthine at a dose of 1000 mg/kg,except for the normal group.Blood samples were collected for the assay of serum biochemical parameters 45 minutes after the injection.The animals were then sacrificed,and their livers were harvested for the determination of xanthine oxidase(XOD)activity by a kit.The kidneys were also collected for RNA extraction,and after reverse transcription,the mRNA expression levels of urate transporter 1(URAT1)and organic anion transporter 1(OAT1)were determined by quantitative real-time PCR.Results The serum uric acid(UA)level and liver XOD activity of the model group were higher than that of the normal group,the mRNA expression level of URAT1 in the kidney was higher than that of the normal group,while the mRNA expression level of OAT1 was lower than that of the normal group,the differences were statistically significant(P＜0.01 or P＜0.001).Administration of 200 mg/kg and 400 mg/kg of GAS decreased serum UA of the mice.Meanwhile,they also reduced liver XOD activity,decreased renal URAT1 mRNA expression level and increased that of OAT1(P＜0.05 or P＜0.01 compared to model group).ALLO restored the above parameters to normal(P＜0.01 or P＜0.001 compared to model group).Conclusion GAS improves acute HUA of mice induced by hypoxanthine,which may be attributable to inhibition of liver XOD activity,down-regulation of renal URAT1 mRNA expression,and up-regulation of renal OAT1 mRNA expression.

Key words： Gastrodin Hyperuricemia Allopurinol Xanthine oxidase Urate transporter 1 Organic anion transporter 1

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	CHU Ya-hui
	KONG Wei-jia

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CHU Ya-hui,KONG Wei-jia. Study of the effects and mechanisms of gastrodin on acute hyperuricemia of mice induced by hypoxanthine[J]. 中国当代医药, 2021, 28(26): 23-26.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2021/V28/I26/23

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