Objective To analyze the clinical effect of vitamin B12 injection at Zusanli acupoint in the treatment of diabetic peripheral neuropathy.Methods A total of 60 patients with diabetic peripheral neuropathy admitted to Leping Hospital of Traditional Chinese Medicine from January 2020 to January 2022 were selected.They were divided into the control group and the observation group by random number table methods,with 30 cases in each group.The control group received regular monitoring,diet and exercise intervention on the basis of drug control of blood glucose,while the observation group was given vitamin B12 injection at Zusanli acupoint on the basis of conventional treatment intervention.The changes of blood glucose level,treatment effective rate and adverse reactions before and after treatment were compared between the two groups,and statistical analysis was conducted.Results The levels of fasting blood glucose (FPG)and blood glucose 2-hour postprandial glucose (2 h PG)in the two groups after treatment were lower than before treatment,and the levels of FPG and 2 h PG in the observation group after treatment were lower than those in the control group,with statistical significance (P＜0.05).The traditional Chinese Medicine syndrome score of the two groups after treatment was lower than that before treatment,and the traditional Chinese Medicine syndrome score of the observation group after treatment was lower than that of the control group,with statistical significance (P＜0.05).The motor conduction velocity and sensory conduction velocity of common peroneal nerve,plantar nerve and tibial nerve in the two groups after treatment were higher than those before treatment,and the motor conduction velocity and sensory conduction velocity of common peroneal nerve,plantar nerve and tibial nerve in the observation group after treatment were higher than those in the control group,with statistical significance(P＜0.05).The effective rate of the observation group was significantly higher than that of the control group,with statistical significance (P＜0.05).There was no significant difference in the incidence of adverse reactions between the two groups (P＞0.05).Conclusion Zusanli acupoint injection of vitamin B12 in the treatment of diabetic peripheral neuropathy has reliable clinical effect,which is helpful to control the blood sugar level of patients,reduce the traditional Chinese Medicine symptom score,improve the motor nerve and sensory nerve conduction velocity,and improve the clinical therapeutic effect.The treatment method is simple,safe,and worthy of clinical promotion and application.