Abstract：Objective To establish the quality standard of Tiangui Congying Granules.Methods Cistanche deserticola,lily,artemisia anomala and fruit of glossy privet in Tiangui Congying Granules were identified by thin layer chromatography(TLC).High performance liquid chromatography-diode-array detector (HPLC-DAD)was used to simultaneously determine the contents of echinacin and ligustrin.Acetonitrile -0.1% phosphoric acid as mobile phase for gradient elution,and the detection wavelength were 330 nm(echinacea)and 224 nm(ligustrin)respectively.And the column temperature was 35℃.Injection volume was 10 μl.Results The spots in TLC identification chromatogram were clear,the resolution was good,and the negative chromatogram had no interference.The linear relationship between the injection volume and the peak area of echinacin was good between 0.104 0 μg and 2.600 μg(r=0.999 9).The linear relationship between the injection volume and the peak area of ligustroside was good between 0.122 6 μg and 3.065 0 μg(r=1.000 0).Echinacin and ligustrin of the average sample recovery were 97.52% and 104.44% respectively,the RSD were less than 3.0%.Conclusion The established quality standard method has strong specificity,good stability,high accuracy and good durability,and it is used for the quality control of Tiangui Congying Granules.
京公网安备 11010502046607号