Abstract：Objective To establish the thin layer chromatography (TLC) method for identification of compound Sanhuang Gel.Methods The TLC was used to investigate the influence of different developing agent,different temperature and different thin layer plate manufacturers on the identification of rhubarb,scutellaria and phellodendron in compound Sanhuang Gel,and to determine the best TLC conditions.Results The rhubarb was made of silica gel H thin layer plate with toluene-ethyl acetate-formic acid(7∶3∶0.1)as the development agent,removed,dried,examined under 365 nm UV lamp,the spots showed clear color.Polyamide film was used in scutellaria baicalensis,and toluene-ethyl acethane-methanol-formic acid (10∶3∶1∶2)was used as development agent,removed,dried,examined under 365 nm UV lamp,and fumed in ammonia vapor,the spots showed clear color.The silica gel G thin layer plate was used and toluene-methanol-ethyl-acetate-isopropyl-alcohol-concentrated ammonia test solution(6∶1.5∶3∶1.5∶0.5)was used as the development agent,removel,dried,and examined under 365 nm ultraviolet lamp,the spots showed clear color.The TLC of the three drugs showed obvious characteristics,good separation and no negative interference.Conclusion The TCL method is specific and durable,which can be used for the quality control of compound Sanhuang Gel.
甘灿云； 俞 进； 陈海红； 倪天宇； 杨海燕. 复方三黄凝胶薄层色谱鉴别方法研究[J]. 中国当代医药, 2024, 31(15): 9-13.
GAN Canyun YU Jin CHEN Haihong NI Tianyu YANG Haiyan. Study on thin layer chromatography identification of compound Sanhuang Gel. 中国当代医药, 2024, 31(15): 9-13.
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