Objective To investigate the effect of Atosiban on pregnancy outcome of frozen embryo transfer (FET) in patients with implant failure.Methods A total of 408 FET cycles in Nanchang Reproductive Hospital from January 2021 to March 2022 were retrospectively analyzed.Based on pregnancy outcomes,patients was divided into Atosiban group(208 cases) and non-Atosiban group (200 cases).The general information and assisted reproduction outcome of the two groups were statistically analyzed.According to the number of transplantation failures,the patients were divided into embryo transfer (ET)＜1 and ET≥1 for subgroup analysis.Results There were no significant differences in age,primary infertility rate,infertility years,body mass index,endometrial thickness,type A endometrial ratio,number of transplanted embryos,clinical pregnancy rate,spontaneous abortion rate and ectopic pregnancy rate between Atosiban group and non-Atosiban group (P＞0.05).The results of subgroup analysis showed∶for patients with ET≥1,there were no significant differences in endometrial thickness,type A endometrial ratio,the number of embryo transfer,the rate of spontaneous abortion and ectopic pregnancy rate between Atosiban group and non-Atosiban group (P＞0.05),and the clinical pregnancy rate of Atosiban group was higher than non-Atosiban group,the difference was statistically significant (P＜0.05).For patients with ET＜1,there were no statistical significances in all the above indicators between the two groups (P＞0.05).Conclusion Atosiban can significantly improve the pregnancy outcome of FET patients with implantation failure,and there was no affect on the spontaneous abortion rate and ectopic pregnancy rate,which is a good treatment for the pregnancy outcome of patients with implantation failure.