Objective To explore the application effect of Double-Subject scheme in teaching practice of nursing interns.Methods A total of 380 nursing interns who were interned in Jiangxi Province Children′s Hospital from June 2019 to July 2021 were selected as the research objects.According to the order of admission practice,177 nursing students who were interned from June 2019 to March 2020 were set as the control group,and 203 nursing students who were interned from July 2020 to March 2021 were set as the intervention group.The control group used conventional teaching method,and the intervention group used Double-Subject scheme teaching method.The operation and theoretical achievements,clinical communication ability and clinical adaptability of the two groups of nursing students after training were compared.Results The scores of operation assessment and theoretical assessment of the two groups of nursing interns after training were higher than those before training,and the differences were statistically significant(all P＜0.05).The scores of operation assessment and theoretical assessment of nursing interns in the intervention group after training were higher than those in the control group,and the differences were statistically significant(P＜0.05).The scores of harmonious relationship,keen listening,confirming patients′problems,verifying feelings and the total scores of nursing interns in the scores of clinical adaptability in the intervention group were higher than those in the control group,and the differences were statistically significant(P＜0.05).However,there were no statistically significant difference in the scores of common participation dimension and transmitting effective information between the two groups(P＞0.05).The scores of professional cognition and emotion in the clinical adaptability scores of nursing interns in the intervention group were higher than those in the control group,and the differences were statistically significant(P＜0.05).There were no significant difference in the scores of interpersonal relationship,behavioral performance dimension and total score of clinical adaptation between the two groups(P＞0.05).Conclusion Double-Subject scheme teaching method can improve the professional level and communication ability of nursing interns,but it has no obvious effect on improving their clinical adaptability.