Objective To explore the clinical characteristics of acute Entoloma omiense poisoning, and to provide scientific basis for clinical diagnosis, treatment and nursing of this kind of mushroom poisoning in the future. Methods The clinical data of 31 patients with acute Entoloma omiense poisoning treated in Guangzhou Twelfth People′s Hospital from June 2016 to July 2021 were analyzed retrospectively. The clinical characteristics and nursing of poisoning patients were retrospectively analyzed. Results The clinical characteristics of 31 patients were consistent with gastroenteritis type mushroom poisoning. All patients had acute gastroenteritis. The poisoning latency was 20 min to 5 h, with an average of (1.18±1.16) h. The main symptoms were nausea in 31 cases, vomiting in 30 cases, diarrhea in 31 cases and abdominal pain in 19 cases. The increase of peripheral blood leukocyte count in laboratory examination accounted for 93.5%（29/31）. There were no abnormalities in liver function, renal function and myocardial related biochemical indexes. After gastric lavage, adsorption, catharsis, symptomatic support and nursing. All patients recovered in a short time. The hospital stay was 1-4 days, with an average of (2.58±1.06) days. Conclusion Acute Entoloma omiense poisoning is gastroenteritis type, generally does not cause liver and kidney damage, and the prognosis is good. In case of poisonous mushroom poisoning, the types of poisonous mushrooms should be found out as soon as possible and the clinical types of poisonous mushrooms should be clarified. Accurate treatment and nursing measures were taken according to the types of virus causing mushrooms and poisoning types.