P300/CBP相关因子和P53在宫颈癌组织中的表达及其相关性

摘要
图/表
参考文献(20)
相关文章 (15)

全文: PDF (643 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨P300/CBP 相关因子（PCAF）和P53 在宫颈癌组织中的表达情况及二者之间的相关性。方法选取2016年6月至2020年6月东营市东营区人民医院收治的79 例宫颈癌患者组织标本作为实验组，并选取同期20例正常宫颈组织标本作为对照组，应用免疫组织化学SP 法检测PCAF 和P53 在宫颈组织中的表达，比较分析两组PCAF和P53 表达的特点以及相关性。结果实验组的PCAF 阳性表达率为41.8%，低于对照组的80.0%，差异有统计学意义（P<0.05）。实验组的PCAF 阳性表达率随宫颈癌临床分期及病理分级的增高而逐渐降低，差异有统计学意义（P<0.05）。实验组中不同年龄和组织学类型患者的PCAF 阳性表达率比较，差异无统计学意义（P>0.05）。实验组的P53 阳性表达率（78.5%）高于对照组（0%），差异有统计学意义（P<0.05）。实验组的P53 阳性表达率随宫颈癌临床分期及病理分级的增高而逐渐增高，差异有统计学意义（P<0.05）。实验组中不同年龄和组织学类型患者的P53阳性表达率比较，差异无统计学意义（P>0.05）。实验组中PCAF 和P53 的表达成中度负相关（r=-0.493，P<0.05）。结论 PCAF 在宫颈癌中低表达甚至不表达，与P53 蛋白的异常高表达呈负相关，且与宫颈癌的恶性临床病理特征相关，提示PCAF 和P53 在宫颈癌的发生发展中起一定作用，有望成为宫颈癌重要的生物学分子标志物。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李蕾
	张俊涛
	曹淑萍

关键词 ： P300/CBP 相关因子, P53, 宫颈癌, 抑癌基因

Abstract：Objective To explore the expressions of P300/CBP-related factor (PCAF) and P53 in cervical carcinoma and the relationship between the two factors. Methods From June 2016 to June 2020, 79 cervical cancer tissue samples admitted to Dongying District People′s Hospital of Dongying City were selected as the experimental group, and 20 normal cervical tissue samples were selected as the control group. Immunohistochemical SP method was used to detect the expression of PCAF and P53 in cervical tissues. The expression characteristics and correlation of PCAF and P53 were compared between the two groups. Results The positive expression rate of PCAF in the experimental group was 41.8%,which was lower than 80.0% in the control group, and the difference was statistically significant (P<0.05). The positive expression rate of PCAF in the experimental group decreased with the increase of clinical stage and pathology classification of cervical cancer, and the difference was statistically significant (P<0.05). There was no significant difference in the positive expression rate of PCAF among patients in experimental group of different ages and histological types (P>0.05).The positive expression rate of P53 in the experimental group(78.5%)was higher than that in the control group(0%),and the difference was statistically significant (P<0.05). The positive expression rate of P53 in the experimental group increased with the increase of clinical stage and pathology classification of cervical cancer, and the difference was statistically significant (P<0.05). There was no significant difference in the positive expression rate of P53 among patients in experimental group of different ages and histological types(P>0.05).There was a moderate negative correlation between the expression of PCAF and P53 in the experimental group (r=-0.493,P<0.05).Conclusion The expression rate of PCAF in cervical cancer is low or even zero, which is negatively correlated with the active expression of P53. It is suggested that PCAF and P53 may play essential roles in the development of cervical cancer and may become important biological molecular markers for cervical cancer.

Key words： P300/CBP-related factor P53 Cervical carcinoma Anti-oncogene

基金资助:山东省东营市科学技术项目（东科成评字[2021]第305 号）

通讯作者: 曹淑萍（1975-），女，山东寿光人，硕士，副主任医师，研究方向：妇科内分泌。

作者简介: 李蕾（1983-），女，山东禹城人，硕士，研究方向：妇科肿瘤及消化道肿瘤的诊断。

引用本文:

李蕾；张俊涛；曹淑萍. P300/CBP相关因子和P53在宫颈癌组织中的表达及其相关性[J]. 中国当代医药, 2022, 29(27): 25-28.
LI Lei；ZHANG Juntao；CAO Shuping. The expression and correlation of P300/CBP-related factor and P53 in cervical cancer. 中国当代医药, 2022, 29(27): 25-28.

链接本文:

https://www.dangdaiyiyao.com/CN/ 或 https://www.dangdaiyiyao.com/CN/Y2022/V29/I27/25

[1]	Small W Jr，Bacon MA，B ajaj A，et al.Cervical cancer：A global health crisis[J].Cancer，2017，123（13）：2404-2412.
[2]	刘倩，王玮.浅谈宫颈癌临床治疗新进展[J].实用医学杂志，2018，34（1）：5-7.
[3]	He H，Wang J，Liu T.UV-Induced RPA1 Acetylation Promotes Nucleotide Excision Repair[J].Cell Rep，2017，20（9）：2010-2025.
[4]	Sunami Y，Araki M，Kan S，et al.Histone Acetyltransferase p300/CREB-binding Protein-associated Factor （PCAF） Is Required for All-trans-retinoic Acid-induced Granulocytic Differentiation in Leukemia Cells[J].J Biol Chem，2017，292（7）：2815-2829.
[5]	Gai X，Tu K，Li C，et al.Histone acetyltransferase PCAF accelerates apoptosis by repressing a GLI1/BCL2/BAX axis in hepatocellular carcinoma[J].Cell Death Dis，2015，6（4）：e1712.
[6]	Linares LK，Kiernan R，Triboulet R，et al.Intrinsic ubiquitination activity of PCAF controls the stability of the oncoprotein Hdm2[J].Nat Cell Biol，2007，9（3）：331-338.
[7]	Wang T，Yao W，Shao Y，et al.PCAF fine-tunes hepatic metabolic syndrome，inflammatory disease，and cancer[J].J Cell Mol Med，2018，22（12）：5787-5800.
[8]	Kong D，Ying B，Zhang J，et al.PCAF Regulates H3 Phosphorylation and Promotes Autophagy in Osteosarcoma Cells[J].Biomed Pharmacother，2019，118（10）：1-9.
[9]	赵宝忠，侯继申，张乐，等.食管癌Survivin 乙酰化水平与PCAF P300 表达的相关性及临床意义[J].河北医学，2019，25（8）：1377-1380.
[10]	Fei HJ，Zu LD，Wu J，et al.PCAF acts as a gastric cancer suppressor through a novel PCAF-p16-CDK4 axis[J].Am J Cancer Res，2016，6（12）：2772-2786.
[11]	Mazzà D，Infante P，Colicchia V，et al.PCAF ubiquitin Ligase activity inhibits Hedgehog/Gli1 signaling in p53-dependent response to genotoxic stress[J].Cell Death Differ，2013，20（12）：1688-1697.
[12]	Liu T，Wang X，Hu W，et al.Epigenetically Down-Regulated Acetyltransferase PCAF Increases the Resistance of Colorectal Cancer to 5-Fluorouracil[J].Neoplasia，2019，21（6）：557-570.
[13]	Mitildzans A，Arechvo A，Rezeberga D，et al.Expression of p63，p53 and Ki-67 in Patients with Cervical Intraepithelial Neoplasia[J].Turk Patoloji Derg，2017，33（1）：9-16.
[14]	Halle MK，Ojesina AI，Engerud H，et al.Clinicopathologic and molecular markers in cervical carcinoma：a prospective cohort study[J].Am J Obstet Gynecol，2017，217（4）：1-17.
[15]	Zhou R，Wei C，Liu J，et al.The prognostic value of p53 expression for patients with cervical cancer：a meta analysis[J].Eur J Obstet Gynecol Reprod Biol，2015，195：210-213.
[16]	齐青霞，潘莹，何全中，等.羟喜树碱对外源野生型p53转染宫颈癌细胞作用效果的研究[J].肿瘤药学，2019，9（1）：40-44.
[17]	Luff SA，Kao CY，Papoutsakis ET.Role of p53 and transcription-independent p53-induced apoptosis in shearstimulated megakaryocytic maturation，particle generation，and platelet biogenesis[J].PLoS One，2018，13（9）：e0203991.
[18]	Malatesta M，Steinhauer C，Mohammad F，et al.Histone acetyltransferase PCAF is required for Hedgehog-Gli-dependent transcription and cancer cell proliferation[J].Cancer Res，2013，73（20）：6323-6333.
[19]	Lu YF，Xu XP，Lu XP，et al.SIRT7 activates p53 by enhancing PCAF-mediated MDM2 degradation to arrest the cell cycle[J].Oncogene，2020，39（24）：4650-4665.
[20]	Linares LK，Kiernan R，Triboulet R，et al.Intrinsic ubiquitination activity of PCAF controls the stability of the oncoprotein Hdm2[J].Nat Cell Biol，2007，9（3）：331-338.