Objective To analyze the infection status and drug susceptibility of urogenital mycoplasma in Tianjin Second People′s Hospital in the past five years, so as to provide reference for rational clinical diagnosis and treatment.Methods A total of 1448 patients admitted to outpatient or inpatient clinics of Tianjin Second People′s Hospital from January 2017 to December 2021 were selected retrospectively as research objects, and their secretions were tested and analyzed by Mycoplasma culture identification counting drug sensitivity kit. Results Among 1448 patients admitted to the clinic in the past 5 years, the number of positive cases was 406, the total detection rate was 28.04%, and the difference of the annual detection rate was statistically significant (P＜0.001), showing a downward trend year by year. The detection rate of single ureaplasma urealyticum (Uu) was the highest in 5 years (20.93%), followed by mixed infection of Uu and mycoplasma hominis (Mh) (5.59%). The positive detection rate of males was 16.7%, and the positive detection rate of females was 51.93%. There were statistically significant differences between males and females in the total detection rate and the detection rate of a single Uu, Uu+Mh infection type (P＜0.001) , and the age with the highest proportion was 19-30 years old (37.68%). The drug susceptibility results showed that the differences in the drug susceptibility results of different infection types were statistically significant (P＜0.05). But Minocycline, Doxycycline and Josamycin were still effective drugs for the treatment of the disease. Analysis of the susceptibility rate of main antibiotics in 5 years showed that the susceptibility rate of Erythromycin and Clarithromycin had a significant upward trend,and the susceptibility rate of Levofloxacin had an obvious downward trend. Conclusion Uu infection was the main mycoplasma positive infection in urogenital tract, and females are susceptible. There are also differences in the medication for different types of infection, and the medication can be individualized according to the drug susceptibility results.Although Minocycline, Doxycycline and Josamycin are the first-choice drugs for treatment, the sensitivity of these three drugs has declined in the past five years, indicating that drug-resistant strains frequently appear, and the rational use of antibiotics should be standardized in clinical practice.