Correlation analysis of tumor necrosis factor-α, cytokeratin 19 fragment antigen 21-1 expression and disease stage in non-small cell lung cancer
WANG Chenliang1 ZHANG Jing1 LIU Haoru1 LI Guanhua2▲
1. Department of Pathology, the First People′s Hospital of Jiujiang City, Jiangxi Province, Jiujiang 332000, China;
2. Department of Laboratory, the First People′s Hospital of Jiujiang City, Jiangxi Province, Jiujiang 332000, China
Abstract：Objective The correlation between the expression of tumor necrosis factor-α (TNF-α) and cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) in non-small cell lung cancer was analyzed. Methods Sixty patients with non-small cell lung cancer admitted to Jiujiang First People′s Hospital from October 2018 to November 2021 were selected as the research subjects. Cancer tissue and paracancer tissue (more than 5 cm away from cancer tissue) were collected for immunohistochemical staining. The expression levels of CYFRA21-1 and TNF-α were detected by chemiluminescence. Results The positive rate of TNF-α expression and CYFRA21-1 expression in cancer tissues were higher than those in adjacent tissues, and the differences were statistically significant (P<0.05). The positive rate of TNF-α and CYFRA21-1 expression in patients with pathological stage Ⅲ-Ⅳ were higher than those in patients with pathological stage Ⅰ-Ⅱ, and the differences were statistically significant (P<0.05). The positive expression of TNF-α in patients with pathological stage Ⅲ-Ⅳ was stronger than that in patients with pathological stage Ⅰ-Ⅱ, and the difference was statistically significant (P<0.05). The expressions of TNF-α and CYFRA21-1 were positively correlated with pathological stage (P<0.05). Conclusion TNF-α and CYFRA21-1 were abnormally high in non-small cell lung cancer tissues, which were correlated with the pathological stages of the disease.
Banday MZ，Balkhi HM，Hamid Z，et al.Tumor Necrosis Factor-α （TNF-α）-308g/a Promoter Polymorphism in Colorectal Cancer in Ethnic Kashmiri Population-a Case Control Study in a Detailed Perspective-Sciencedirect[J].Meta Gene，2016，9：128-136.
[12]
Zhang S，Wang H，Xu Z，et al.Lymphatic Metastasis of Nsclc Involves Chemotaxis Effects of Lymphatic Endothelial Cells Through the Ccr7-ccl21 Axis Modulated By TNF-α[J].Genes，2020，11（11）：1309.
[13]
Hong QY，Di Z，Yuan YS，et al.Ataxia-telangiectasia Mutated Activation Mediates Tumor Necrosis Factor-alpha Induced Mmp-13 Up-regulation and Metastasis in Lung Cancer Cells[J].Oncotarget，2016，7（38）：62070-62083.
[14]
Zhu J，Xin Y，Liu X，et al.Nimotuzumab enhances the sensitivity of non-small cell lung cancer cells to tumor necrosis factor-α by inhibiting the nuclear factor-κB signaling pathway[J].Exp Ther Med，2018，15：3345-3351.
[15]
Yi F，Shi X，Pei X，et al.Tumor necrosis factor-alpha-308 gene promoter polymorphism associates with survival of cancer patients：A meta-analysis.[J].Medicine （Baltimore），2018，97（5）：e13160.
[16]
Allen H，Shraga-heled N，Blumenfeld M，et al.Human Placental-derived Adherent Stromal Cells Co-induced with TNF-α and IFN-γ Inhibit Triple-negative Breast Cancer in Nude Mouse Xenograft Models[J].Sci Rep，2018，8（1）：670
[17]
Singh PK，Bogra J，Chandra G，et al.Association of Tnf-α （-238 and -308） Promoter Polymorphisms with Susceptibility of Oral Squamous Cell Carcinoma in North Indian Population[J].Cancer Biomark，2015，15（2）：31-125.
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