Mechanism and significance of microRNA-214 targeted regulation of HOXB7 in inhibiting the invasion and metastasis of colorectal cancer cells
LIU Ying1 HE Fengping1▲ LIU Yulan2 LI Dingyun3
1. Physical Examination Center, Yuebei People's Hospital Affiliated to Medical College of Shantou University, Guangdong Province, Shaoguan 512026, China; 2. Clinical Laboratory, Yuebei People's Hospital Affiliated to Medical College of Shantou University, Guangdong Province, Shaoguan 512026, China;
3. Gastrointestinal Surgery, Yuebei People's Hospital Affiliated to Medical College of Shantou University, Guangdong Province, Shaoguan 512026, China
Objective To investigate the mechanism and significance of microRNA-214 (miRNA-214) targeted regulation of HOXB7 to inhibit the invasion and metastasis of colorectal cancer (CRC) cells. Methods A total of 200 inpatients with CRC admitted to Yuebei People's Hospital Affiliated to Medical College of Shantou University from January 2018 to December 2020 were selected as the research subjects, and were divided into non-metastatic group (n=100)and metastatic group (n=100) according to the occurrence of metastasis, and 200 healthy individuals during the same period were selected as the control group. Molecular biological techniques were used to detect the expression of miRNA-214, HOXB7, and Wnt/β-catenin in tissue cells, and the clinical significance was analyzed. Results The expression level of miRNA-214 in the tissues of patients of non-metastasis group and metastasis group were lower than those of the control group, the differences were statistically significant (P<0.05). The expression level of miRNA-214 in CRC SW1116 and SW480 cell lines was lower than that in normal cells, the differences were statistically significant (P<0.05). Transfection of SW1116 and SW480 cells with miRNA-214 mimics showed that high expression of miRNA-214 resulted in low expression of HOXB7. HOXB7 was the target gene of miRNA-214, and the expression level of the two was negatively correlated (r=-0.394, P<0.05). The expression level of HOXB7 in non-metastatic group and metastatic group were higher than those in control group,and the differences were statistically significant (P<0.05). The expression level of HOXB7 in SW1116 and SW480 cell lines were higher than those in normal cells, and the differences were statistically significant (P<0.05). When HOXB7 was overexpressed, Wnt/β-catenin expression was also increased, while when HOXB7 was interfered with by miRNA-214, Wnt/β-catenin expression was significantly decreased. Conclusion The miRNA-214—HOXB7—Wnt/β-catenin pathway is involved in inhibiting the invasion and metastasis of colorectal cancer, which provides a new target for the diagnosis and treatment of colorectal cancer.
刘 英; 何凤屏; 刘玉兰; 李定云. microRNA-214靶向调控HOXB7基因抑制结直肠癌细胞侵袭转移的机制与意义[J]. 中国当代医药, 2022, 29(18): 5-10.
LIU Ying;HE Fengping; LIU Yulan; LI Dingyun. Mechanism and significance of microRNA-214 targeted regulation of HOXB7 in inhibiting the invasion and metastasis of colorectal cancer cells. 中国当代医药, 2022, 29(18): 5-10.
Sun W，Li J，Zhou L.The c-Myc/miR-27b-3p/ATG10 Regulatory Axis Regulates Chemoresistance in Colorectal Cancer[J].Theranostics，2020，10（5）：1981-1996.
[4]
Liu J，Ke F，Chen T.MicroRNAs That Regulate PTEN as Potential Biomarkers in Colorectal Cancer：A Systematic Review[J].J Cancer Res Clin Oncol，2020，146（4）：809-820.
Monterisi S，Lo Riso P，Russo K，et al.HOXB7 overexpression in lung cancer is a hallmark of acquired stem-like phenotype[J].Oncogene，2018，37（26）：3575-3588.
[10]
Liu FT，Chen HM，Xiong Y，et al.Deregulated HOXB7 expression predicts poor prognosis of patients with malignancies of digestive system[J].Minerva Chir，2019，74（5）：422-430.
Zhou Z，Wu L，Liu Z，et al.MicroRNA-214-3p targets the PLAGL2-MYH9 axis to suppress tumor proliferation and metastasis in human colorectal cancer[J].Aging（Albany NY），2020，12（10）：9633-9657.
[15]
Gao D，Chen HQ.Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway[J].Am J Physiol Cell Physiol，2018，315（5）：C675-C686.
Wang R，Sun Y，Yu W，et al.Downregulation of miRNA-214 in cancer-associated fibroblasts contributes to migration and invasion of gastric cancer cells through targeting FGF9 and inducing EMT[J].J Exp Clin Cancer Res，2019，38（1）：20.
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