血清miRNA-187及miRNA-143在胆囊癌患者中的表达及其诊断价值

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摘要目的探讨血清miRNA-187及miRNA-143在胆囊癌患者中的表达及其诊断价值。方法选取2013年9月至2021年3月北部战区总医院收治的92 例胆囊癌患者为胆囊癌组，另取同期收治的80 例胆囊良性疾病患者作为胆囊良性疾病组，再选取同期健康体检者80 例作为健康对照组。采集所有研究对象的血清，检测并比较血清miRNA-187及miRNA-143 水平。绘制ROC 曲线分析血清miRNA-187及miRNA-143 水平诊断胆囊癌的效能。比较不同临床特征胆囊癌患者血清miRNA-187及miRNA-143 的表达情况。结果胆囊癌组血清miRNA-187高于胆囊良性疾病组及健康对照组，而miRNA-143 低于胆囊良性疾病组及健康对照组；胆囊良性疾病组血清miRNA-187 高于健康对照组，而miRNA-143 低于健康对照组，差异有统计学意义（P＜0.05）。血清miRNA-187 联合miRNA-143 诊断胆囊癌的AUC、灵敏度、特异度及约登指数均高于上述两项指标单独诊断。TNM 分期为Ⅲ～Ⅳ期、低分化及淋巴结转移胆囊癌患者的血清miRNA-187 表达水平高于TNM 分期为Ⅰ～Ⅱ期、中高分化及无淋巴结转移胆囊癌患者，差异有统计学意义（P＜0.05）；TNM 分期为Ⅲ～Ⅳ期、低分化及淋巴结转移胆囊癌患者的血清miRNA-143 表达水平均低于TNM 分期为Ⅰ～Ⅱ期、中高分化及无淋巴结转移胆囊癌患者，差异有统计学意义（P＜0.05）。结论血清miRNA-187 在胆囊癌中异常高表达，而miRNA-143 异常低表达，联合检测可能有助于胆囊癌的临床诊断以及病情评估。

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关键词 ：胆囊癌, MiRNA-187, MiRNA-143, 诊断价值, 临床病理特征

Abstract：Objective To study expression and diagnostic value of serum miRNA-187 and miRNA-143 in patients with gallbladder cancer.Methods A total of 92 patients with gallbladder cancer admitted to General Hospital of Northern Theater Command from September 2013 to March 2021 were selected as the gallbladder cancer group.In addition,80 patients with benign gallbladder disease treated at the same period were selected as the benign gallbladder disease group,and 80 healthy subjects at the same period were selected as the healthy control group.Serum of all subjects was collected,and serum levels of miRNA-187 and miRNA-143 were detected and compared.ROC curve was drawn to analyze the diagnostic efficacy of serum miRNA-187 and miRNA-143 levels in gallbladder cancer.The expressions of serum miRNA-187 and miRNA-143 in patients with gallbladder carcinoma with different clinical characteristics were compared.Results Serum miRNA-187 in gallbladder cancer group was higher than that in benign gallbladder disease group and healthy control group,while miRNA-143 was lower than that in benign gallbladder disease group and healthy control group,serum miRNA-187 in benign gallbladder disease group was higher than that in healthy control group,while miRNA-143 was lower than that in healthy control group,the differences were statistically significant (P<0.05).The AUC,sensitivity,specificity and Youden index of serum miRNA-187 combined with miRNA-143 in the diagnosis of gallbladder carcinoma were higher than those of the above two indexes alone.The expression level of miRNA-187 in serum of patients with TNM stage Ⅲto Ⅳ,poorly differentiated and lymph node metastasis was higher than that of patients with TNM stage Ⅰto Ⅱ,moderately well-differentiated and no lymph node metastasis,the differences were statistically significant (P<0.05).The expression level of miRNA-143 in serum of patients with TNM stageⅢto Ⅳ,poorly differentiated and lymph node metastasis gallbladder cancer was lower than that of patients with TNM stage Ⅰto Ⅱ,moderately well-differentiated and no lymph node metastasis gallbladder cancer,the differences were statistically significant (P<0.05).Conclusions Serum miRNA-187 is abnormally high in gallbladder cancer,while miRNA-143 is abnormally low.Combined detection may be helpful for clinical diagnosis and disease evaluation of gallbladder cancer.

Key words： Gallbladder cancer MiRNA-187 MiRNA-143 Diagnostic value Clinicopathological characteristics

引用本文:

葛欣. 血清miRNA-187及miRNA-143在胆囊癌患者中的表达及其诊断价值[J]. 中国当代医药, 2022, 29(14): 96-99.
GE Xin. Expression and diagnostic value of serum miRNA-187 and miRNA-143 in patients with gallbladder cancer. 中国当代医药, 2022, 29(14): 96-99.

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https://www.dangdaiyiyao.com/CN/ 或 https://www.dangdaiyiyao.com/CN/Y2022/V29/I14/96

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