Abstract:Objective To study the protective mechanism of Baicalin on the myocardium of diabetic rats. Methods Twenty-four male SD rats were randomly divided into the control group (6 rats), the Baicalin control group (6 rats), the model group (6 rats) and the treatment group (6 rats). Experimental diabetes models were established by tail vein injection of 45 mg/kg streptozotocin in the model group and the treatment group. The Baicalin control group and the treatment group were given intraperitoneal injection of 15 μmol/(L·kg·d) Baicalin liquid, while in the control group and the model group, the intraperitoneal injection of normal saline was used. The four groups were fed with normal diet. After 8 weeks, blood glucose, mitochondrial structure, myocardial mitochondrial damage ratio, activity of respiratory chain complexes Ⅰ, Ⅱ, Ⅲ, Ⅳ, ND1 mRNA and ND1 protein expression were compared among the four groups. Results The blood glucose of the model group was higher than that of the control group, and the blood glucose of the treatment group was lower than that of the model group, the differences were statistically significant (P<0.05). The percentage of damaged mitochondria in the model group was higher than that in the control group, and the percentage of damaged mitochondria in the treatment group was lower than that in the model group, and the differences were statistically significant (P<0.05). The activities of myocardial mitochondrial respiratory chain complexes Ⅰ, Ⅱ, Ⅲ, Ⅳin the model group were lower than those in the control group, the activities of myocardial mitochondrial respiratory chain complexes Ⅰ,Ⅱ, Ⅲ, Ⅳin the treatment group were higher than those in the model group, the differences were statistically significant (P<0.05). The ND1 mRNA and ND1 protein in the model group were lower than those in the control group, and the ND1 mRNA and ND1 protein in the treatment group were higher than those in the model group, the differences were statistically significant (P<0.05). Conclusion The activity of mitochondrial respiratory chain complex and the expression of ND1 gene and protein of mitochondrial respiratory chain complex Ⅰsubunit were decreased in diabetic rats. Baicalin may play a protective role in diabetic myocardium by increasing the activity of mitochondrial respiratory chain complex and enhancing the expression of ND1 gene and protein of complex Ⅰsubunit.
Ighodaro OM.Molecular pathways associated with oxidative stress in diabetes mellitus Osasenaga Macdonald Ighodaro[J].Biomed Pharmacother,2018,108:656-662.
[2]
Jiang CH,Moorthy BT,Patel DM,et al.Regulation of Mitochondrial Respiratory Chain Complex Levels,Organization,and Function by Arginyltransferase[J].Front Cell Dev Biol,2020,8:603688.
Yang J,Yang X,Yang J,et al.Baicalin ameliorates lupus autoimmunity by inhibiting differentiation of Tfh cells and inducing expansion of Tfr cells[J].Cell Death Dis,2019,10(2):140-146.
[7]
Ma LY,Wu F,Shao QQ,et al.Baicalin Alleviates Oxidative Stress and Inflammation in Diabetic Nephropathy via Nrf2 and MAPK Signaling Pathway[J].Drug Des Devel Ther,2021,15:3207-3221.
[8]
Zhang Y,Taufalele PV,Cochran JD,et al.Mitochondrial Pyruvate Carriers are Required for Myocardial Stress Adaptation Nat Metab[J].Nat Metab,2020,2(11):1248-1264.
[9]
Taylor SC,Nadeau K,Abbasi M,et al.The Ultimate qPCR Experiment:Producing Publication Quality,Reproducible Data the First Time[J].Trends Biotechnol,2019,37(7):761-774.
[10]
Xie Z,Wu B,Shen G,et al.Curcumin alleviates liver oxidative stress in type 1 diabetic rats[J].Mol Med Rep,2018,17(1):103-108.
[12]
Brown DA,Perry JB,Allen ME,et al.Mitochondrial function as a therapeutic target in heart failure[J].Nat Rev Cardiol,2017,14(4):238-250.
[13]
Cunnane SC,Trushina E,Morland C,et al.Brain energy rescue:an emerging therapeutic concept for neurodegenerative disorders of ageing[J].Nat Rev Drug Discov,2020,19(9):609-633.
[14]
Yang SK,Han YCH,Liu J,et al.Mitochondria:A Novel Therapeutic Target in Diabetic Nephropathy[J].Curr Med Chem,2017,24(29):3185-3202.
Ma YY,Li XY,Li ZQ,et al.Clinical,biochemical,and genetic analysis of the mitochondrial respiratory chain complex Ⅰdeficiency[J].Medicine,2018,97(32):e11606.
[18]
Wang H,Ma XD,Cheng QB,et al.Deep Eutectic Solvent-Based Ultrahigh Pressure Extraction of Baicalin from Scutellaria baicalensis Georgi[J].Molecules,2018,23(12):3233.
[20]
Liu XL,Zhang SSH,Xu CY,et al.The Protective of Baicalin on Myocardial Ischemia-Reperfusion Injury[J].Curr Pharm Biotechnol,2020,21(13):1386-1393.
[21]
Wu J,Chen H,Qin JH,et al.Baicalin Improves Cardiac Outcome and Survival by Suppressing Drp1-Mediated Mitochondrial Fission after Cardiac Arrest-Induced Myocardial Damage[J].Oxid Med Cell Longev,2021,2021:8 865 762.