LI Ya′nan1 JIN Chunming2▲ GONG Fangyan2 GAO Yu1 DENG Meng1 ZHANG Hang1 DING Xinzhe1 YIN Guibin1
1. The First School of Clinical Medicine, Mudanjiang Medical University, Heilongjiang Province, Mudanjiang 157000,China;
2. Department of Laboratory Medicine, Hongqi Hospital Affiliated to Mudanjiang Medical University, Heilongjiang Province, Mudanjiang 157000, China
Objective To investigate the effect of microRNA-501-5p(miR-501-5p)in breast cancer.Methods Forty patients with breast cancer diagnosed for the first time in Tumor Hospital of Mudanjiang City from March 2020 to March 2021 were selected as the breast cancer group. Forty physical examination subjects in Hongqi Hospital Affiliated to Mudanjiang Medical University during the same period were selected as the healthy control group. Serum of patients in the two groups was collected, and the expression level of miR-501-5p was detected by real-time fluorescence quantitative PCR (qRT-PCR). The expression of LAMTOR5 in tissues was detected by immunohistochemistry. ROC curve was drawn to analyze the value of serum miR-501-5p, carcinoembryonic antigen (CEA), carbohytrate antigen 153(CA153) and carbohytrate antigen 125 (CA125) in the diagnosis of breast cancer. Results The expression level of miR-501-5p in breast cancer tissue (1.12±0.23) was higher than that in adjacent tissues (0.96±0.15), and the difference was statistically significant (P<0.05). The expression level of miR-501-5p in serum of breast cancer patients (0.96±0.67)was higher than that of healthy controls (0.88±0.12), and the difference was statistically significant (P<0.05). The expression level of miR-501-5p in patients with lymph node metastasis and TNM stage Ⅲand Ⅳwere higher than those in patients with TNM stage Ⅰand Ⅱwithout lymph node metastasis, and the differences were statistically significant (P<0.05). The positive rate of LAMTOR5 expression in breast cancer tissue was higher than that in adjacent tissues, and the difference was statistically significant (P<0.05). The AUC of serum miR-501-5p, CEA, CA153 and CA125 in the diagnosis of breast cancer was 0.663, 0.827, 0.687 and 0.633,respectively, and the AUC of four combined diagnosis of breast cancer was 0.877. Conclusion The expression of miR-501-5p is increased in breast cancer tissues and serum of breast cancer patients,and its expression is related to lymph node metastasis and clinical TNM stage of breast cancer.The positive expression of LAMTOR5 in breast cancer tissues was higher than that in adjacent tissues.The expression of LAMTOR5 was positively correlated with the expression level of miR-501-5p. Combined detection of miR-501-5p, CEA, CA153 and CA125 can improve the diagnostic level of breast cancer.
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