[Abstract] Objective To use modern disease prevention and control theory to predict and evaluate disease trends, explore the epidemic trend and prevention and control measures of hand food and mouth disease (HFMD). Methods A total of 4373 cases of hand, foot and mouth disease in Yingkou city of from 2016 to 2018 were selected to analyze the incidence and discuss prevention and control measures. Results Among 4373 cases of hand, foot and mouth disease patients were divided into peak incidence in July, accounting for 41.98% of the total number of cases. The patients were mainly children, with 3989 cases, accounting for 91.22% of the total number of cases. Among them, there were 2127 children in kindergartens, accounting for 48.64% of the total incidence, and 1862 cases of scattered children, accounting for 42.58% of the total incidence. The minimum age of onset was 1 month, and the maximum age was 71 years old.The age of onset was mainly 1-4 years old, with 3184 cases, accounting for 72.81% of the total incidence number. The cases were mainly distributed in 1390 cases in Bayuquan area, accounting for 31.79%. Case pharyngeal swabs and stool samples were collected by fluorescence quantitative RT-PCR method to detect enterovirus nucleic acid, the positive samples were also virus-separated, positive for 865 copies. In 2016, it was mainly of the CoxA16 type, 80.60% of positive detection in 2016; in 2017, mainly with type EV71, 95.90% of the positive detection in 2017; in 2018, other types of enteroviruses, 70.04% of positive detection in 2018. The incidence of HFMD was compared with pathogens from 2008 to 2018, morbidity and other universal primer rank correlation analysis showed that, there was a negative correlation between incidence and other universal primer positive rates (r=-0.736, P<0.05). Conclusion According to the three distribution characteristics of HFMD cases and the pathogen test results, Hand, foot and mouth disease mostly occurs mostly in children aged 1-4, July reached its peak,when other general primers are high, the incidence is declining. Before the epidemic period, targeted HFMD prevention and control strategies were formulated to achieve better prevention and control results.