Effect and mechanism of Dabuyuan Decoction on ovarian reserve function based on FOXO3a mediated PI3K/AKT pathway
WANG Yong DENG Gaopi▲ HUANG Ya′nan WU Xiaomin
Department of Gynecology, the Affiliated Traditional Chinese Medicine Hospital of Guangzhou Medical University, Guangdong Province, Guangzhou 510000, China
Abstract：Objective To investigate the effect and mechanism of Dabuyuan Decoction medicated serum on the improvement of ovarian reserve hypofunction through the phosphatidylinositol 3-kinases (PI3K)/protein kinase B (AKT) signaling pathway mediated by FOXO3a. Methods Thirty-six healthy SD rats were randomly divided into blank group (n=6), model group (n=6), Daybuyuan Decoction high-dose group (n=6), Daybuyuan Decoction medium-dose group (n=6), Daybuyuan Decoction low-dose group (n=6) and Western medicine group (n=6), both were injected with Cyclophosphamide 75 mg/kg to make the model. After corresponding treatment, the ovarian reserve function of each group was compared. Results HE staining results showed that, compared with the model group, the number of follicles, the thickness of granular layer, the number of layers, and the degree of arrangement density of ovarian tissue in the Daybuyuan Decoction high-dose, medium-dose and low-dose groups and the Western medicine group were significantly increased, while the number of atresia follicles was decreased. Serum levels of estradiol (E2), anti-Müllerian hormone (AMH) and inhibin B (INH-B) in blank group were higher than those in other groups, while serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in blank group were lower than those in other groups, the differences were statistically significant (P<0.05). Serum E2, AMH and INH-B in Daybuyuan Decoction high-dose, medium-dose and low-dose groups and Western medicine group were higher than those in model group, while FSH and LH were lower than those in model group, the differences were statistically significant (P<0.05). Serum E2, AMH and INH-B in Daybuyuan Decoction high-dose group were higher than those in Daybuyuan Decoction medium-dose and low-dose groups and Western medicine group, while FSH and LH were lower than those in Daybuyuan Decoction medium-dose and low-dose groups and Western medicine group, the differences were statistically significant (P<0.05). There were no statistically significant differences in serum E2, AMH, INH-B, FSH and LH of Daybuyuan Decoction medium-dose and low-dose groups and Western medicine group (P>0.05). The expression level of FOXO3a in blank group and Daybuyuan Decoction high-dose group was lower than that in other groups, while the expression of PI3K and AKT were higher than those in other groups, with statistical significances (P<0.05). There were no significant differences in FOXO3a, PI3K and AKT expression levels between blank group and Daybuyuan Decoction high-dose group (P>0.05). The expression level of FOXO3a in Daybuyuan Decoction high-dose, medium-dose and low-dose groups and Western medicine group were lower than those in model group, with statistical significances (P<0.05). There were no significant differences in FOXO3a, PI3K and AKT expression levels among Daybuyuan Decoction medium-dose group, low-dose group and Western medicine group (P>0.05). Conclusion Dabuyuan Decoction can consolidate the body, nourish qi and blood, and has a significant effect in regulating the decline of ovarian function. It has the effect of alleviating the symptoms of premature ovarian failure in rats. This effect is closely related to the mechanism of regulating FOXO3a, PI3K and AKT expression levels in vivo.
王勇； 邓高丕； 黄亚南； 吴晓敏. 基于FOXO3a介导的PI3K/AKT信号通路研究大补元煎改善卵巢储备功能作用及机制[J]. 中国当代医药, 2022, 29(4): 4-8.
WANG Yong； DENG Gaopi； HUANG Ya′nan； WU Xiaomin. Effect and mechanism of Dabuyuan Decoction on ovarian reserve function based on FOXO3a mediated PI3K/AKT pathway. 中国当代医药, 2022, 29(4): 4-8.
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