Establishment of non-alcoholic fatty liver model in type 2 diabetic rats
ZHAO Yan1 TAN Hong1 LI Huifang1 LYU Qionghua1 DU Sicheng2 YANG Qiuping2▲
1. Department of Diabetes, the First Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650032, China;
2. Department of Geriatrics, the First Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650032, China
Abstract：Objective To establish a non-alcoholic fatty liver model in rats with type 2 diabetes, and to explore the effect of type 2 diabetes on the liver of rats. Methods Forty healthy male SD rats were randomly divided into normal control group (NC) and diabetic group (DM), with 20 rats in each group. Type 2 diabetic rats in DM group were induced by intraperitoneal injection of streptozotocin (STZ, 35 mg/kg) combined with high-glucose and high-fat diet. After modeling, blood glucose, triacylglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), liver index, insulin sensitive index (ISI), Homeostasismodel assessment insulin resistance index (HOMA-IR) related indicators were detected. Liver tissue of rats was stained with HE and its pathological changes were observed. Results At 6, 7, 8, 9, 10 weeks after modeling, the body weight of DM group was lower than that of NC group, and blood glucose was higher than that of NC group, the differences were statistically significant (P<0.05). TG, TC, ALT and liver index in DM group were higher than those in NC group, the differences were statistically significant (P<0.05). There was no significant difference in AST between the two groups (P>0.05). 6, 10 weeks after modeling, the fasting insulin (FINS), HOMA-IR of DM group were higher than those of NC group, ISI was lower than that of NC group, the differences were statistically significant (P<0.05). Under the light microscope, the normal liver cells in NC group were orderly, the liver lobules were normal, the cytoplasm was round and uniform, and there was no infiltration of steatosis and inflammatory cells. In DM group, liver cells were improperly organized, fatty degeneration, liver cell edema, infiltration of inflammatory cells, partial spots and local necrosis. The activity score of non-alcoholic fatty liver disease (NAS) in DM group was higher than that in NC group, and the difference was statistically significant (P<0.05). Conclusion Intraperitoneal injection of low-dose STZ combined with high-sugar and high-fat feeding can induce a non-alcoholic fatty liver model of type 2 diabetes in rats.
赵燕； 谭洪； 李会芳； 吕琼花； 杜思成； 杨秋萍. 2型糖尿病大鼠非酒精性脂肪肝模型的建立[J]. 中国当代医药, 2022, 29(20): 48-51.
ZHAO Yan； TAN Hong； LI Huifang； LYU Qionghua； DU Sicheng； YANG Qiuping. Establishment of non-alcoholic fatty liver model in type 2 diabetic rats. 中国当代医药, 2022, 29(20): 48-51.
Zheng Y，Ley SH，Hu FB.Global aetiology and epidemiology of type 2 diabetes mellitus and its complications[J].Nat Rev Endocrinol，2018，14（2）：88-98.
[2]
Wang L，Gao P，Zhang M，et al.Prevalence and Ethnic Pattern of Diabetes and Prediabetes in China in 2013[J].JAMA，2017，317（24）：2515-2523.
[3]
Li Y，Teng D，Shi X，et al.Prevalence of diabetes recorded in mainland China using 2018 diagnostic criteria from the American Diabetes Association：national cross sectiona study[J].BMJ，2020，369：m997.
[6]
Khneizer G，Rizvi S，Gawrieh S.Non-alcoholic Fatty Liver Disease and Diabetes Mellitus[J].Adv Exp Med Biol，2021， 1307：417-440.
[7]
Guo Z，Zhang R，Li J，et al.Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats[J].Cardiovasc Di abetol，2012，11：94.
Diehl AM，Day C.Cause，pathogenesis，and treatment of nonalcoholic steatohepatitis[J].N Engl J Med，2017，377（21）：2063-2073.
[12]
Younossi ZM，Koenig AB，Abdelatif D，et al.Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence，incidence，and outcomes[J].Hepatology，2016，64（1）：73-84.
[13]
Wong SW，Chan WK.Epidemiology of non-alcoholic fatty liver disease in Asia[J].Indian J Gastroenterol，2020，39（10）：1-8.
[14]
Parameswaran M，Hasan HA，Sadeque J，et al.Factors That Predict the Progression of Non-alcoholic Fatty Liver Disease （NAFLD）[J].Cureus，2021，13（12）：e20776.
[15]
Wasilewska N，Lebensztejn DM.Non-alcoholic fatty liver disease and lipotoxicity[J].Clin Exp Hepatol，2021，7（1）：1-6.
[16]
Milovanovic T，Pantic I，Dragasevic S，et al.The Interrelationship Among Non-Alcoholic Fatty Liver Disease，Colonic Diverticulosis and Metabolic Syndrome[J].J Gastrointestin Liver Dis，2021，30（2）：274-282.
Chalasani N，Younossi Z，Lavine JE，et al.The diagnosis and management of nonalcoholic fatty liver disease：Practice guidance from the American Association for the Study of Liver Diseases[J].Hepatology，2018，67（1）：328-357.
京公网安备 11010502046607号