Abstract：Objective To explore the effect of miR-34a-Sirt1-Nrf2 signal pathway on the proliferation of endogenous neural stem cells after cerebral ischemia injury.Methods Rats with modified focal middle cerebral artery embolism(MCAO) cerebral ischemia were prepared，and they were set as cerebral ischemia 1 d group(n=10)，cerebral ischemia 3 d group (n=10)，cerebral ischemia 7 d group (n=10)，resveratrol group (n=10)，and rats without ischemic treatment were also set up as control group (n=10).The expression levels of mir-34a，Sirt1，NRF2 in the subventricular zone of rats in each group were detected by RT-PCR.Results Compared with the control group，the relative expression of miR-34a mRNA in the resveratrol group，cerebral ischemia 1 d group，cerebral ischemia 3 d group and cerebral ischemia 7 d group increased，while the relative expression of Sirt1 and NRF2 protein decreased，the differences were statistically significant (P＜0.05).Compared with the resveratrol group，the relative expression of miR-34a mRNA in the cerebral ischemia 1 d group，the cerebral ischemia 3 d group and the cerebral ischemia 7 d group increased，while the relative expression of Sirt1 and NRF2 protein decreased，the differences were statistically significant (P＜0.05).Compared with the cerebral ischemia 1 d group，the relative expression of miR-34a mRNA in the cerebral ischemia 3 d group and the cerebral ischemia 7 d group increased，and the relative expression of Sirt1 and NRF2 protein decreased，the differences were statistically significant (P＜0.05).Compared with the cerebral ischemia 3 d group，the relative expression of miR-34a mRNA in the cerebral ischemia 7 d group increased，while the relative expression of Sirt1 and NRF2 protein decreased，and the differences were statistically significant(P＜0.05).Conclusion miR-34a may regulate the proliferation of endogenous neural stem cells after cerebral ischemic injury by regulating Sirt1/NRF2 pathway.
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