Study on the inhibitory effect of XPO4 gene on human liver cancer cells
WU Nan1，2 LIU Kan2 WANG Xiao-mei2
1.Department of Disease Prevention，Nanshan District Center for Disease Control and Prevention of Shenzhen City，Guangdong Province，Shenzhen 518054，China;
2.Institute of Oncology，Medical Science Department of Shenzhen University，Guangdong Province，Shenzhen 518000，China
Abstract：Objective To construct a vector expressing tumor suppressor gene XPO4 and observe its inhibitory effect on liver cancer cells.Methods The XPO4 gene was inserted into the eukaryotic expression vector pcDNA3.1 to construct the tumor suppressor gene expression vector pcDNA3.1-XPO4.Transfection of hepatocellular carcinoma cell line SKHep1 was performed in vitro.The XPO4 gene expression was observed by Western Blot，and tumor cell inhibition was detected by cell counting kit-8(CCK-8)and Annexin V/PI.Results The pcDNA3.1-XPO4 plasmid vector constructed in this study was transfected into SK-Hep1 cells in vitro，and can effectively promote the expression of tumor suppressor gene XPO4 in cells.The cell survival rate of the recombinant plasmid PCDNA3.1-XPO4 transfection group was lower than that of the SK-Hep1 cell control group and the pcDNA3.1 empty plasmid transfection group，and the differences were statistically significant (P＜0.001).The early apoptosis rate of the recombinant plasmid pcDNA3.1-XPO4 transfection group ([13.89±1.62]%) was higher than that of the SK-Hep1 cell control group ([5.14±1.13]%) and the pcDNA3.1 empty plasmid transfection group ([5.79±2.11]%)，and the differences were statistically significant (P＜0.001).Conclusion XPO4 tumor suppressor gene expression can significantly inhibit the proliferation of SK-Hep1 tumor cells and induce cell apoptosis，indicating that XPO4 may play an important role in the occurrence and development of hepatocellular carcinoma，and may become a potential method for diagnosis and treatment of hepatocellular carcinoma.
吴 楠；刘 侃；王晓梅. XPO4基因对人肝癌细胞抑制作用的研究[J]. 中国当代医药, 2020, 27(33): 17-20.
WU Nan；LIU Kan；WANG Xiao-mei. Study on the inhibitory effect of XPO4 gene on human liver cancer cells. 中国当代医药, 2020, 27(33): 17-20.
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