Expression and distribution of GAD-65/67 in 23 patients with epileptogenic forcal cortical dysplasia
RAO Ying-hua1 ZHANG Xun1 QIN Ming-jun1 HU Bin2
1.Department of Neurosurgery,the Affiliated Brain Hospital of Guangzhou Medical University(Guangzhou Huiai Hospital),Guangdong Province,Guangzhou 510370,China;
2.Department of Neurosurgery,the Affiliated Second Hospital of Guangzhou Medical University,Guangdong Province,Guangzhou 510500,China
Abstract：Objective To explore the expression and distribution of GAD-65/67 in patients with forcal cortical dysplasia(FCD)epilepsy.Methods Surgical focus specimen of inpatients were collected from the Affiliated Brain Hospital of Guangzhou Medical University and the Affiliated Second Hospital of Guangzhou Medical University from October 2017 to October 2019.The expression of GAD-65/67 was observed by immunohistochemical staining in 23 patients with FCD epilepsy who underwent surgical resection (the experimental group)and 7 normal brain tissues without epilepsy(the control group).All the stained sections were examined under a microscope and photographed with a digital camera and the image chromatic aberration was analyzed by IEE 7.0 software.Results The expression of GAD-65/67 was found in both the experimental group and the control group.In the control group,GAD-65/67 was found to be evenly distributed in nerve fibers and synapses in brain tissue.In the experimental group,abnormal morphology and arrangement of neurons were observed and the expression and distribution of GAD-65/67 were changed correspondingly.Conclusion This study suggests that the abnormal distribution of GAD-65/67 may be attributed to the abnormal development of cerebral cortex in FCD,resulting in the decrease of GABA inhibition.
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