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| Feasibility of laser confocal microscope observation of tumors at different inoculation sites |
| PIAO Ying DU Rong YU Han-zhi JIANG Xiang-hong |
| College of Chemical and Biological Engineering, Bio -nano Engineering Center, Zhejiang University, Hangzhou 310012, China |
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Abstract Objective To compare the feasibility and applicability of laser confocal microscoe for in situ observation of drug intratumoral micro-behavior in mice with abdominal subcutaneous tumors and ear subcutaneous tumors, so as to provide a better method for in situ observation of drug penetration in tumors.Methods The murine breast cancer cell line 4T1 transfected with green fluorescent protein (GFP) (4T1-GFP) was cultured in vitro.4T1-GFP cells were subcutaneously injected into the abdominal subcutaneous vein or ear vein of female ICR mice aged 6-8 weeks.When the tumors grow to 4-7 mm3, the mice would be operated on and the tumors will be pasted on the slide.Cy5-labeled nanodrugs were injected into mice via tail vein, and programmed image acquisition was performed by confocal laser microscope.Results The optimal concentration of tumor cells was (2-3)×104/μl for ear tumor inoculation, the tumors could be used for experiments from the second day.And the optimal concentration of tumor cells was (1-2)×104/μl for abdominal subcutaneous inoculation, the tumors could be used for experiments from the third day.Tumors with less vascular branches and disc-like growth were more conducive to drug-mediated angiosmosis imaging.The model of ear and abdominal subcutaneous tumors could well show the process of drug penetration.Conclusion The models of ear and abdominal subcutaneous tumors have their own advantages and disadvantages in drug vascularization.Both models provide a new method for the study of drug vascularization in tumors.
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