三种ABO 血型检测方法的临床应用

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Abstract Objective To discuss the accuracy and influence factors of ABO blood type detected by microcolumn gel test (MGT), manual polybrene test (MPT) and slide method. Methods Blood identification samples of 3000 patients admitted to our hospital from December 2017 to June 2018 were analyzed, and ABO blood type was detected by MGT,MPT and slide method. Re-examination rate of blood group test results of three blood group detection methods was compared. The samples with suspicious blood group results were re-examined by adding reverse typing, and the factors affecting ABO blood type detection were analyzed. Results Among the 3000 samples, 57 cases were found to need to be re-examined for blood group results. According to the re-examination rate, MGT (0.17%)＜MPT (0.37%)＜slide method (1.37%). There was no significant difference in the rate of blood group re-examination between MGT and MPT(χ2=2.274, P=0.68551). There was a significant difference in the re-examination rate of blood group results between slide method and MPT method (χ2=18.378, P=0.00104), there was a significant difference in the re-examination rate of blood group results between MGT and slide method (χ2=29.556, P=0.00001). The influencing factors of blood type test results of three methods were as follows： slide method (70.7%)＞MPT (27.3%)＞MGT (20.0%)； equipment factor： MGT(80.0%)＞MPT (9.1%)＞Slide Method (7.3%)； operation factor： MPT (63.6%)＞Slide Method (22.0%)＞MGT (0.0%). Conclusion There are some deficiencies in three ABO blood group detection methods, the accuracy of MGT is higher than that of MPT and slide method. It is suggested that the combination of positive and negative typing be used to compensate for the deficiency of positive typing and provide accurate ABO blood type results for clinical transfusion treatment.

Key words： Microcolumn gel test Manual polybrene test Slide method Blood type identification Re-examination rate

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	PAN Guo-xiong
	LIU Zhi-lin
	HE Jia-ying
	XIONG Liu-xin

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PAN Guo-xiong,LIU Zhi-lin,HE Jia-ying, et al. Clinical application of three kinds of ABO blood type detection method[J]. 中国当代医药, 2019, 26(9): 161-163转180.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2019/V26/I9/161

[1]	崔颖，杜娟，杨世明，等.多发性骨髓瘤患者血型鉴定和交叉配血的影响因素及处理方法[J].细胞与分子免疫学杂志，2017，33（10）：1419-1421.
[2]	Zhong Q，Yin WM，Yang-Ling OU.Screening and identification of ABO blood group antigens by microcolumn gel and serological techniques[J].J Trop Med，2018，12（1）：40-42.
[3]	Bhattacharya P，Samanta E，Afroza N，et al.An approach to incompatible cross-matched red cells：our experience in a major regional blood transfusion center at Kolkata，Eastern India[J].Asian J Transfus Sci，2018，12（1）：51-56.
[4]	Kinnunen EM，Zanobini M，Onorati F，et al.The impact of minor blood transfusion on the outcome after coronary artery by pass grafting[J].J Crit Care，2017，40：207-212.
[5]	Lee HY，Park MJ，Kim NY，et al.Rapid direct PCR for ABO blood typing[J].J Forensic Sci，2011，56（1）：179-182.
[6]	彭道波，兰炯采，王梁平等．用微柱凝胶试验进行交叉配血[J]．中国输血杂志，2001，14（4）：232.
[7]	谢霞，张勇萍，杨世明，等.13 例高球蛋白血症对ABO 血型鉴定和交叉配血结果的干扰及处理方法[J].细胞与分子免疫学杂志，2017，33（6）：835-837.
[8]	王培华．输血技术学[M]．北京：人民卫生出版社，2002：152-152．
[9]	Lalezari P，Jiang AF.The manual polybrene test：a simple and rapid procedure for detection of red cell antibodies[J].Transfusion，2010，20（2）：206-211.
[10]	江涛，张勇萍，杨世明，等.血标本纤维蛋白析出对血型鉴定和交叉配血及抗体筛查的影响[J].国际检验医学杂志，2017，38（20）：2885-2887.
[11]	谢霞，张勇萍，杨世明，等.13 例高球蛋白血症对ABO 血型鉴定和交叉配血结果的干扰及处理方法[J].细胞与分子免疫学杂志，2017，33（6）：835-837.
[12]	Dubinski D，Won SY，Behmanesh B，et al.Influence of ABO blood type on the outcome after non-aneurysmal subarachnoid hemorrhage[J].Acta Neurochir （Wien），2018，160（4）：761-766.
[13]	张萍，陈荇.常见ABO 血型鉴定错误原因分析[J].检验医学与临床，2009，6（1）：75-76.
[14]	Foley C L，Mould T，Kennedy J E，et al. A study of blood cross-matching requirements for surgery in gynecological oncology：improved efficiency and cost saving[J].Int J Gynecol Cancer，2010，13（6）：889-893.
[15]	Guo K L，Guan-Hua OU.Research of pre-blood transfusion test and blood transfusion strategy for newborns[J].Am J Chin Med，2017，26（13）：54-55.
[16]	Goulet S，Giger U，Arsenault J，et al.Prevalence and mode of inheritance of the Dal blood group in dogs in north America[J].J Vet Intern Med，2017，31（3）：751-758.