不同时段尿标本检测巨细胞病毒在小儿人巨细胞病毒感染中的应用

Abstract
Figure/Table
References (15)
Related Citation (15)

Download: PDF (0 KB) HTML (30 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To investigate the effect of fluorescent quantitative PCR in detection of cytomegalovirus(HCMV)in urine samples of children with HCMV infection at different time intervals.Methods A total of 30 cases of infection in our hospital from September 2015 to February 2017 in Department of Pediatrics of infected children,for all children were collected in the morning urine as morning urine group,collecting urine specimens as random specimens from other time periods,the application of real-time fluorescence quantitative PCR technology in different periods of urine HCMV DNA level standard for examination,the positive detection rate was compared between the two groups.Results The results showed that morning urine group,27 cases were detected HCMV positive,the detection rate of 90.00%,random time urine group,20 cases were detected HCMV positive,the detection rate of 66.67%,compared the two groups,morning urine group was significantly higher than that of random time group,the difference was statistically significant(P<0.05).Conclusion The detection of HCMV real-time fluorescent quantitative PCR in children with infection of HCMV in children,collection of morning urine samples for testing,the positive rate is high,effectively reduce the misdiagnosis,it is worth in clinical application.

Key words： Human cytomegalovirus Children Real-time quantitative PCR Different periods

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HUANG Bin
	LIU Su-yun
	WAN Li-jia
	LIU Ming
	WU Xiao-yang
	LUO Qin

Cite this article:

HUANG Bin,LIU Su-yun,WAN Li-jia, et al. Detection of cytomegalovirus in children with cytomegalovirus infection by urine samples at different time intervals[J]. 中国当代医药, 2018, 25(12): 119-121.

URL:

http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2018/V25/I12/119

[1]	翁玉英，倪琛，林秀凤，等.不同标本巨细胞病毒检测在诊断小儿人巨细胞病毒感染中的应用[J].分子诊断与治疗杂志 2014，6（6）：410-414.
[2]	Cameron BM，Kennedy SE，Rawlinson WD，et al.The efficacy of valganciclovir for prevention of infections with cytomegalovirus and Epstein-Barr virus after kidney transplant in children[J].Pediatr Transplant，2017，21（1）：145-146.
[3]	Rovito R，Korndewal MJ，Schielen PCJI，et al.Neonatal screeningparameters in infants with congenital Cytomegalovirus infection[J].Clin Chim Acta，2017，13（19）：226-228.
[4]	余鹃春，王左，项贵明，等.实时荧光定量PCR方法对3种不同标本中巨细胞病毒检出率的比较[J].医学研究杂志，2012，41（2）：134-136.
[5]	吉耀华，羿永东，李书琴，等.尿标本池检测法筛选新生儿先天性人巨细胞病毒感染[J].中国医科大学学报，2013，42（6）：535-538.
[6]	Verma K，Palaniswamy G，Cremonini G，et al.Late cytomegalovirus infection in children：High incidence of allograft rejection and hepatitis in donor negative and seropositive liver transplant recipients[J].Pediatr Transplant，2017，21（3）：23-24.
[7]	高培杰，赖惠英，傅清流.荧光定量PCR检测巨细胞病毒感染的标本选择[J].中华医院感染学杂志，2011，21（10）：2149-2151.
[8]	刘燕琼，秦雪，彭契六，等.人巨细胞病毒感染与婴儿肝炎综合征及肝功能损害的相关性研究[J].重庆医学，2014，43（36）：4903-4905.
[9]	谢康，刘艳秋，阳彦，等.母乳、小儿血液及尿液标本中检测巨细胞病毒对诊断小儿HCMV感染的临床应用[J].实验与检验医学，2017，35（3）：340-343.
[10]	边鹏，温凤，韩兆香，等.59例小儿巨细胞病毒感染IL-2检测分析[J].吉林医学，2014，35（24）：5341-5342.
[11]	袁浩，唐曾，周毅峰.巨细胞病毒的3种检测方法在诊断小儿巨细胞病毒感染中的评价[J].现代预防医学，2014，41（14）：2607-2610.
[12]	谢康，刘艳秋，阳彦，等.母乳、小儿血液及尿液标本中检测巨细胞病毒对诊断小儿HCMV感染的临床应用[J].实验与检验医学，2017，35（3）：340-343.
[13]	钟瑞芬，黎四平，梁丽云，等.尿HCMV-DNA检测在新生儿疾病中的应用价值[J].国际检验医学杂志，2016，37（4）：475-477.
[14]	王泽民.小儿巨细胞病毒（HCMV）感染对患儿造血系统的影响观察[J].内蒙古医科大学学报，2015，37（S2）：17-19.
[15]	王敬衍，程丽敏，钱冬萌，等.不同标本巨细胞病毒DNA检测在小儿巨细胞感染中的应用[J].世界最新医学信息文摘，2016，16（80）：131-132.