Abstract：Objective To explore the effect of indirect counting method (MPN method) in GB 4789.3-2016 "National Food Safety Standard for food microbiology test—Coliforms Count" on detecting coliforms in water.Methods The water samples were inciuded urban drinking water, rural drinking water, reservoir water, and water source from Fengcheng city from October 2017 to April 2018.The coliforms in water were detected by the MPN method in GB 4789.3-2016 and the total coliforms MPN method in GB/T 5750.12-2006 "Standard examination methods for drinking water—Microbilolgical parameters", separately.The correlation between the two methods for detecting coliform bacteria was verified.The manpower, material resources, cost, time and the positive rate of coliform bacteria detected by the two methods were compared.Results The consistency of two MPN methods to detect coliform group in water quality was good(Kappa=0.97).There was no significant difference in the positive rate of coliform detected by two MPN methods (P＞0.05).The instrument cost, reagent cost and labor cost of food coliform group MPN method were lower than those of water coliform group MPN method, and the consumption time was shorter than that of water coliform group MPN method, and the differences were statistically significant (P＜0.05).Conclusion MPN method of food coliform group can replace MPN method of water coliform group to detect coliform group of water quality, which can greatly save manpower, material and financial resources.
Zhu L，Torres M，Betancourt WQ，et al.Incidence of fecal indicator and pathogenic bacteria in reclaimed and return flow waters in Arizona USA[J].Environ Res，2018，170：122-127.
[6]
Kaushik S，Tiwari UK，Pal SSL，et al.Rapiddetection of Escherichia coliusing fiber optic surface plasmon resonance immunosensor based on biofunctionalized Molybdenum disulfide（MoS2）na[J].Biosens Bioelectron，2019，126：501-509.
[7]
Clausen CH，Dimaki M，Bertelsen CV，et al.Bacteria detection and differentiation using impedance flow cytometry[J].Sensors（Basel），2018，18（10）：E3496.
Ghilamicael AM，Boga HI，Anami SE，et al.Potential human pathogenic bacteria in five hot springs in Eritrea revealed by next generation sequencing[J].PLoS One，2018，13（3）：e0194554.
[11]
Gallego A，Laurino Soulé J，Napolitano H，et al.Biodegradability of chlorophenols in surface waters from the urban area of buenos aires[J].Bull Environ Contam Toxicol，2018，100（4）：541-547.
[12]
Gutiérrez-Del-Río I，Marín L，Fernández J，et al.Development of a biosensor protein bullet as a fluorescentmethodfor fastdetectionofEscherichia coliin drinkingwater[J].PLoS One，2018，13（1）：e0184277.
[13]
Muniesa M，Ballesté E，Imamovic L，et al.Bluephage：a rapidmethodfor thedetectionof somatic coliphages used as indicators of fecal pollution inwater[J].Water Res，2018，128：10-19.
[14]
Ezenarro JJ，Uria N，Castillo-Fernández O，et al.Development of an integratedmethodof concentration and immunodetection of bacteria[J].Anal Bioanal Chem，2018，410（1）：105-113.
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