Determination of the content of cardiac glycoside in Zhuang herb Streptocaulon griffithii and its influencing factors of different producing areas and harvesting seasons
LUO Yu-dong1,2,3 TAN An-qiang1▲ LU Shi-heng1 WU Yu-qiang1,3 ZHANG Qiang1
1.Pharmaceutical Factory,Guangxi University of Chinese Medicine,Nanning 530023,China;
2.Key Laboratory of Zhuang Medical Formulae and Applied Research of Colleges and Universities in Guangxi Zhuang Autonomous Region,Nanning 530001,China;
3.Key Laboratory of Common Technology Research and Development of Traditional Chinese Medicine Preparation of Colleges and Universities in Guangxi Zhuang Autonomous Region,Nanning 530001,China
Abstract：Objective To establish a method for the determination of cardiac glycoside in Zhuang herb Streptocaulon griffithii,and to study the influence of different producing areas and harvesting seasons on the content of medicinal herb.Methods The colorimetric reaction of α,β -unsaturated lactone ring of type A cardiac glycosides was used to establish the method for the determination of the content of cardiac glycoside in Streptocaulon griffithii by ultraviolet spectrophotometry (UV spectrophotometry).The methodology was also verified.The content of cardiac glycoside in Zhuang herb Streptocaulon griffithii from different producing areas and harvesting seasons was determined.Results By UV spectrophotometry,Digoxin was used as reference substance,and alkaline picric acid solution was used as chromogenic agent.The good linear relation of the content of reference substance and absorbance was displayed in the range of 0-0.08 mg/ml in 415 nm wavelength.The regression equation y=14.968x+0.008 (r=0.9969).The precision of RSD was 0.1%and average recovery was 95.51%(n=6;RSD=2.16%).The average content of cardiac glycosides from 6 batches of Streptocaulon griffithii was 2.07 mg/g.The cardiac glycosides of Streptocaulon griffithii from four different producing areas and seasons in the Guangxi Zhuang Autonomous Region were determined.The highest content of cardiac glycoside of Streptocaulon griffithii was from Basaigou,Qinzhou City and was collected from October to December yearly.Conclusion The established method for the determination of cardiac glycosides of Streptocaulon griffithii has high stability and repeatability in good recovery,and can be used as quality control for Streptocaulon griffithii.Differentproducing areasand harvesting seasons have significant impact on the content of cardiac glycosides of Streptocaulon griffithii.
罗宇东; 谭安蔷; 陆施衡;吴玉强;张强. 壮药藤苦参中强心苷的含量测定及不同产地、采收季节对药材含量的影响[J]. 中国当代医药, 2018, 25(12): 14-17.
LUO Yu-dong;TAN An-qiang; LU Shi-heng;WU Yu-qiang;ZHANG Qiang. Determination of the content of cardiac glycoside in Zhuang herb Streptocaulon griffithii and its influencing factors of different producing areas and harvesting seasons. 中国当代医药, 2018, 25(12): 14-17.
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