Study on gene polymorphism of MSP-1 and MSP-2 of Plasmodium falciparum in Bioko Island in Equatorial Guinea
CHEN Jiang-tao1,2,3 LI Jian4 PAN Xue-feng3 Urbano Monsuy Eyi5 Rocio Apicante Matesa5 Maximo Miko Ondo Obono5 Carlos Sala Ehapo5 ZHA Guang-cai6 HUANG Guang6 HUANG Zhi-xiu6 LIN Min1▲
1.Department of histology and Embryology,Medical College of Shantou University in Guangdong Province,Shantou 515041,China;
2.Laboratory Medicine Center,Center People′s Hospital of Huizhou City in Guangdong Province,Huizhou 516008,China;
3.China′s Medical Detachment of Equatorial Guinea Guangdong Province,Guangzhou 518000,China;
4.School of Basic Medicine in Hubei Medical College,Shiyan 442000,China;
5.Medical Laboratory,Malabo Regional Hospital in Equatorial Guinea,Malabo 999115,Africa;
6.College of Life Science and Food Science And Technology of Hanshan Normal University in Guangdong Province,Chaozhou 521041,China
Abstract：ObjectiveTo study the type of Plasmodium falciparum MSP-1(PfMSP-1)and PfMSP-2 gene in Bioko Island of Equatorial Guinea.Methods181 blood samples were collected from patients with malaria in Bioko Island.Microscopy and fluorescence quantitative PCR were used to identify the insect species.Nested PCR was used to amplify the specific fragment of PfMSP-1 and PfMSP-2 respectively,then the allele typing was conducted.ResultsPfMSP-1 genotypes:178 (98.34%)PfMSP-1 gene fragments were amplified from the 181 blood samples.MAD20-type,K1-type and RO33-type alleles were obtained from 171(94.48%),175(96.69%)and 126(69.61%)samples respectively.The mixed infection rate was 97.24%.PfMSP-2 genotypes:173(95.58%)PfMSP-2 gene fragments were amplified from the 181 blood samples of patients. Single 3D7-type and single FC27-type alleles were obtained in 8(26.52%)and 4(2.21%)samples respectively.The mixed infection rate was 66.85%.ConclusionPfMSP-1 and PfMSP-2 from Bioko Island are widespread.Mixed infection is the main type of malaria in Bioko Island.
陈江涛;李健;潘雪峰;Urbano Monsuy Eyi;Rocio Apicante Matesa;Maximo Miko Ondo Obono. 赤道几内亚比奥科岛恶性疟原虫MSP-1 和MSP-2基因多态性研究[J]. 中国当代医药, 2017, 24(13): 9-12.
CHEN Jiang-tao;LI Jian;PAN Xue-feng;Urbano Monsuy Eyi;Rocio Apicante Matesa;Maximo Miko Ondo Obono;Carlos Sala Ehapo;ZHA Guang-cai;HUANG Guang;HUANG Zhi-xiu;LIN Min. Study on gene polymorphism of MSP-1 and MSP-2 of Plasmodium falciparum in Bioko Island in Equatorial Guinea. 中国当代医药, 2017, 24(13): 9-12.
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